▼Purpose & Project
研究目的
大脳皮質は、記憶、情動、思考、加えて行動など、全ての中枢神経活動を司る。大脳皮質の神経活動は、二種類の構成要素である興奮性神経細胞と抑制性神経細胞のバランスの上に成り立っていている。抑制性神経細胞の働きは、神経活動のバランスを取るのみではなく、適時その抑制を緩めることに
より、興奮性神経細胞の活動を高める。大脳皮質が中枢神経系の頂点にある様に、抑制性神経細胞は興奮性神経細胞の活動を支配し、興奮性神経細胞を支配する抑制性神経細胞は、他の抑制性神経細胞により支配され、全体として階層性支配様式を取っている。本教室では、中枢神経系の頂点に立つ大脳皮質抑制性神経細胞を多角的に理解すべく、分子生物学的、発生学的、形態学的観点から、その多様性と神経活動制御様式の研究を進めている。
研究プロジェクト
1． 大脳皮質GABA作動性神経細胞の産生及び分化 その多様性を生み出す分子メカニズム
2．大脳皮質興奮性神経細胞の産生
3．大脳皮質構成神経細胞数の比率を調節する分子メカニズム
4．投射型大脳皮質GABA作動性神経細胞の働き
5．胎児期および成体脳の神経幹細胞の分化制御機構
6．グリア細胞の脳高次機能における役割
▼Recent Activity
▼Cortical Development in Chania [Invited talk]2011年5月19－22日
▼Publications
2016
Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate,
lateral and ventromedial hypothalamic nuclei.
Sokolowski K, Tran T, Esumi S, Kamal Y, Oboti L, Lischinsky J, Goodrich M,
Lam A, Carter M, Nakagawa Y, Corbin JG.
Neural Dev. 2016 May 21;11(1):
Neural stem cells and neuro/gliogenesis in the central nervous system:
understanding the structural and functional plasticity of the developing,
mature,
and diseased brain.
Yamaguchi M, Seki T, Imayoshi I, Tamamaki N, Hayashi Y, Tatebayashi Y,
Hitoshi S.
J Physiol Sci. 2016 May;66(3):197-206.
Altered proliferative ability of neuronal progenitors in PlexinA1 mutant
mice.
Andrews WD, Davidson K, Tamamaki N, Ruhrberg C, Parnavelas JG.
J Comp Neurol. 2016 Feb 15;524(3):518-34.
2015
Corticofugal GABAergic projection neurons in the mouse frontal cortex.
Tomioka R, Sakimura K, Yanagawa Y.
Front Neuroanat. 2015 Oct 28;9:133
Altered proliferative ability of neuronal progenitors in PlexinA1 mutant
mice.
Andrews WD, Davidson K, Tamamaki N, Ruhrberg C, Parnavelas JG.
J Comp Neurol. 2015 May 15. doi: 10.1002/cne.23806. [Epub ahead of print]PubMed
Glutamate Decarboxylase 67 Deficiency in a Subset of GABAergic Neurons
Induces Schizophrenia-Related Phenotypes.
Fujihara K, Miwa H, Kakizaki T, Kaneko R, Mikuni M, Tanahira C, Tamamaki N, Yanagawa Y.
Neuropsychopharmacology. 2015 Apr 23. doi: 10.1038/npp.2015.117. [Epub
ahead of print]PubMed
Specification of select hypothalamic circuits and innate behaviors by the
embryonic patterning gene dbx1.
Sokolowski K, Esumi S, Hirata T, Kamal Y, Tran T, Lam A, Oboti L, Brighthaupt SC, Zaghlula M,
Martinez J, Ghimbovschi S, Knoblach S, Pierani A, Tamamaki N, Shah NM, Jones KS, Corbin JG.
Neuron. 2015 Apr 22;86(2):403-16. doi: 10.1016/j.neuron.2015.03.022. Epub
2015 Apr 9.PubMed
Contribution of parvalbumin and somatostatin-expressing GABAergic neurons
to slow oscillations and the balance in beta-gamma oscillations across
cortical layers.
Kuki T, Fujihara K, Miwa H, Tamamaki N, Yanagawa Y, Mushiake H.
Front Neural Circuits. 2015 Feb 3;9:6. doi: 10.3389/fncir.2015.00006. eCollection
2015.PubMed
2014
Mammalian-specific sequences in pou3f2 contribute to maternal behavior.
Nasu M, Yada S, Igarashi A, Sutoo D, Akiyama K, Ito M, Yoshida N, Ueda S.
Genome Biol Evol. 2014 Apr 7;6(5):1145-56
2013
Birthdate study of GABAergic neurons in the lumbar spinal cord of the glutamic
acid decarboxylase 67-green fluorescent protein knock-in mouse.
Huang J, Chen J, Wang W, Wei YY, Cai GH, Tamamaki N, Li YQ, Wu SX.
Front Neuroanat. 2013 Dec 9;7:42. doi: 10.3389/fnana.2013.00042. eCollection
2013.PubMed
Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated
sudden death in a mouse model of Dravet syndrome.
Ogiwara I, Iwasato T, Miyamoto H, Iwata R, Yamagata T, Mazaki E, Yanagawa
Y,Tamamaki N, Hensch TK, Itohara S, Yamakawa K.
Hum Mol Genet. 2013 Aug 6. [Epub ahead of print] PubMed
Voltage drives diverse endocannabinoid signals to mediate striatal microcircuit-specific
plasticity.
Mathur BN, Tanahira C,Tamamaki N, Lovinger DM. Nat Neurosci. 2013 Jul 28. [Epub ahead of print] PubMed
Limk2 mediates semaphorin signalling in cortical interneurons migrating
through the subpallium.
Andrews WD, Zito A, Memi F, Jones G, Tamamaki N, and Parnavelas JG.
Biol Open. 2013 15;2(3):277-282.PubMed
Amygdala kindling induces nestin expression in the leptomeninges of the
neocortex.
Shogo Ninomiya, Shigeyuki Esumi, Kunimasa Ohta, Takaichi Fukuda, Tetsufumi Ito, Itaru Imayoshi, Ryoichiro
Kageyama, Toshio Ikeda, Shigeyoshi Itohara, and Nobuaki Tamamaki
Neurosci Res. 2013 75:121-129.PubMed
2012
Robust formation and maintenance of continuous stratified cortical
neuroepithelium by laminin-containing matrix in mouse ES cell culture.
Nasu M, Takata N, Danjo T, Sakaguchi H, Kadoshima T, Futaki S, Sekiguchi K,
Eiraku M, Sasai Y.
PLoS One. 2012;7(12):e53024
Identification of optogenetically activated striatal medium spiny neurons
by npas4 expression.
Bepari AK, Sano H, Nobuaki Tamamaki, Nambu A, Tanaka KF, Takebayashi H.
PLoS One 2012;7(12):e52783 PubMed
Visualization of odor-induced neuronal activity by immediate early gene
expression.
Bepari AK, Watanabe K, Yamaguchi M, Nobuaki Tamamaki, and Hirohide Takebayashi
BMC Neurosci. 2012 13, 140. PubMed
Role of motoneuron-derived neurotrophin 3 in survival and axonal projection
of sensory neurons during neural circuit formation.
Noriyoshi Usui, Keisuke Watanabe, Katsuhiko Ono, Koichi Tomita, Nobuaki Tamamaki, Kazuhiro Ikenaka and Hirohide Takebayashi
Development 2012 139,1125-1132. PubMed
2011
Dpy19L1, a multi-transmembrane protein, regulates radial migration of glutamatergic
neurons in the developing cerebral cortex.
Keisuke Watanabe, Hirohide Takebayashi, Asim K. Bepari, Shigeyuki Esumi, Yuchio Yanagawa and Nobuaki Tamamaki
Development 2011 138, 4979-4990. PubMed
Tangential migration and proliferation of intermediate progenitors of GABAergic
neurons in the mouse telencephalon
Shengxi Wu, Shigeyuki Esumi, Keisuke Watanabe, Jing Chen, Kouichi C.Nakamura, Kazuhiro Nakamura, Kouhei
Kometani, Nagahiro Minato, Yuchio Yanagawa, Kaori Akashi, Kenji Sakimura,
Takeshi Kaneko and Nobuaki Tamamaki.
Development 2011 138, 2499-2509 PubMed
2010
Long-range GABAergic connections distributed throughout the neocortex and
their possible function.
Tamamaki, N. & Tomioka, R.
Front Neurosci. Focused Review 4: 202, 2010 PubMed
Cortical GABAergic Neurons: Stretching it Remarks, Main Conclusions and
Discussion.
Clancy B, Defelipe J, Espinosa A, Fairen A, Jinno S, Kanold P, Luhmann
HJ, Rockland KS, Tamamaki N, Yan XX.
Front Neuroanat.4:7, 2010. PubMed
Draxin, a repulsive axon guidance protein, is involved in hippocampal development.
Zhang S, Su Y, Shinmyo Y, Islam SM, Naser IB, Ahmed G, TamamakiN, Tanaka H＊
Neuroscience research 66: 53-61, 2010. PubMed
2009
Subtypes of GABAergic neurons project axons in the neocortex.
Higo S, Akashi K, Sakimura K, Tamamaki N.
Front Neuroanat. 3: 25 2009. PubMed
Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons
within the spinal dorsal horn of GAD(67)-GFP knock-in mice.
Wang YY, Wei YY, Huang J, Wang W, Tamamaki N, Li YQ, Wu SX.
J Chem Neuroanat. 38(1): 75-81, 2009. PubMed
Parvalbumin neurons in the forebrain as revealed by parvalbumin-Cre transgenic
mice.
Tanahira C, Higo S, Watanabe K, Tomioka R, Ebihara S, Kaneko T, Tamamaki N.
Neurosci Res. 63(3): 213-223, 2009. PubMed
2008
Rapid integration of young newborn dentate gyrus granule cells in the adult
hippocampal circuitry.
Ide Y, Fujiyama F, Okamoto-Furuta K, Tamamaki N, Kaneko T, Hisatsune T
Eur J Neurosci. 28: 2381-2392, 2008. PubMed
Noradrenergic excitation of a subpopulation of GABAergic cells in the basolateral
amygdala via both activation of nonselective cationic conductance and supression
of resting K+ conductance: A study using GAD67-GFP knock-in mice.
Kaneko K, Tamamaki N, Owada H, Kakizaki T, Kume N, Totsuka M, Yamamoto T, Yawo H, Yagi T, Obata
K, Yanagawa Y.
Neuroscience. 154(4): 781-797, 2008. PubMed
Excess Salt Causes Cerebral Neuronal Apoptosis and Inflammation in Stroke-Prone
Hypertensive Rats Through Angiotensin II-Induced NADPH Oxidase Activation.
Yamamoto E, Tamamaki N, Nakamura T, Kataoka K, Tokutomi Y, Dong YF, Fukuda M, Matsuba S, Ogawa
H, Kim-Mitsuyama S.
Stroke. 39(11): 3049-3056, 2008. PubMed
5-HT(3A) receptor subunit is expressed in a subpopulation of GABAergic
and enkephalinergic neurons in the mouse dorsal spinal cord.
Huang J, Wang YY, Wang W, Li YQ, Tamamaki N, Wu SX.
Neurosci Lett. 441(1): 1-6, 2008. PubMed
The protocadherin-alpha family is involved in axonal coalescence of olfactory
sensory neurons into glomeruli of the olfactory bulb in mouse.
Hasegawa S, Hamada S, Kumode Y,Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T.
Mol Cell Neurosci. 38(1): 66-79, 2008. PubMed
Novel strategy to selectively label excitatory and inhibitory neurons in
the cerebral cortex of mice.
Basu K, Gravel C, Tomioka R, Kaneko T,Tamamaki N, Sik A.
J Neurosci Methods. 170(2): 212-219, 2008. PubMed
Method for single-cell microarray analysis and application to gene-expression
profiling of GABAergic neuron progenitors.
Esumi S, Wu SX, Yanagawa Y, Obata K, Sugimoto Y, Tamamaki N.
Neurosci Res. 60(4): 439-451, 2008. PubMed
2007
Anomaly with no right ventral root at the seventh cervical segment in humans:
gross anatomical and neuroanatomical study.
Higo S, Koizumi M, Kawai K, Honma S, Tokiyoshi A, Tamamaki N, Kodama K.
Anat Sci Int. 82(3): 133-138, 2007. PubMed
Long-range GABAergic projection neurons in the cat neocortex.
Higo S, Udaka N, Tamamaki N.
J Comp Neurol. 503(3): 421-431, 2007. PubMed
2006
Dorsally derived netrin 1 provides an inhibitory cue and elaborates the
'waiting period' for primary sensory axons in the developing spinal cord.
Watanabe K, Tamamaki N, Furuta T, Ackerman SL, Ikenaka K, Ono K.
Development. 133(7): 1379-1387, 2006. PubMed
In vivo function of Rnd2 in the development of neocortical pyramidal neurons.
Nakamura K, Yamashita Y, Tamamaki N, Katoh H, Kaneko T, Negishi M.
Neurosci Res. 54(2): 149-153, 2006. PubMed
Split single-cell RT-PCR analysis of Purkinje cells.
Esumi S, Kaneko R, Kawamura Y, Yagi T.
Nat Protoc. 1(4): 2143-2151, 2006. PubMed
Allelic gene regulation of Pcdh-alpha and Pcdh-gamma clusters involving
both monoallelic and biallelic expression in single Purkinje cells.
Kaneko R, Kato H, Kawamura Y, Esumi S, Hirayama T, Hirabayashi T, Yagi T.
J Biol Chem. 281(41): 30551-30560, 2006. PubMed
Predominant expression of rTERTb, an inactive TERT variant, in the adult
rat brain.
Kaneko R, Esumi S, Yagi T, Hirabayashi T.
Protein Pept Lett. 13(1): 59-65, 2006. PubMed
2005
Pyramidal neurons of upper cortical layers generated by NEX-positive progenitor
cells in the subventricular zone.
Wu SX, Goebbels S, Nakamura K, Nakamura K, Kometani K, Minato N, Kaneko
T, Nave KA, Tamamaki N.
Proc Natl Acad Sci U S A. 102(47): 17172-17177, 2005. PubMed
Monoallelic yet combinatorial expression of variable exons of the protocadherin-alpha
gene cluster in single neurons.
Esumi S, Kakazu N, Taguchi Y, Hirayama T, Sasaki A, Hirabayashi T, Koide T, Kitsukawa
T, Hamada S, Yagi T.
Nat Genet. 37(2): 171-176, 2005. PubMed
Developmental pluripotency of the nuclei of neurons in the cerebral cortex
of juvenile mice.
Osada T, Tamamaki N, Song SY, Kakazu N, Yamazaki Y, Makino H, Sasaki A, Hirayama T, Hamada
S, Nave KA, Yanagimachi R, Yagi T.
J Neurosci. 25(37): 8368-8374, 2005. PubMed
Mechanisms of neuropeptide Y, peptide YY, and pancreatic polypeptide inhibition
of identified green fluorescent protein-expressing GABA neurons in the
hypothalamic neuroendocrine arcuate nucleus.
Acuna-Goycolea C, Tamamaki N, Yanagawa Y, Obata K, van den Pol AN.
J Neurosci. 25(32): 7406-7419, 2005. PubMed
Demonstration of long-range GABAergic connections distributed throughout
the mouse neocortex.
Tomioka R, Okamoto K, Furuta T, Fujiyama F, Iwasato T, Yanagawa Y, Obata K, Kaneko
T, Tamamaki N.
Eur J Neurosci. 21(6): 1587-1600, 2005. PubMed
Neurogenesis in the ependymal layer of the adult rat 3rd ventricle.
Xu Y, Tamamaki N, Noda T, Kimura K, Itokazu Y, Matsumoto N, Dezawa M, Ide C.
Exp Neurol. 192(2): 251-264, 2005. PubMed
Origin of the neocortical subependymal cells speculated by Emx1 and GAD67
expression.
Tamamaki N.
Chem Senses. 30 Suppl 1: i111-112, 2005. PubMed
2003
Green fluorescent protein expression and colocalization with calretinin,
parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
Tamamaki N, Yanagawa Y, Tomioka R, Miyazaki J, Obata K, Kaneko T.
J Comp Neurol. 467(1): 60-79, 2003. PubMed
Changes of immunocytochemical localization of vesicular glutamate transporters
in the rat visual system after the retinofugal denervation.
Fujiyama F, Hioki H, Tomioka R, Taki K, Tamamaki N, Nomura S, Okamoto K, Kaneko T.
J Comp Neurol. 465(2): 234-249, 2003. PubMed
Differential distribution of vesicular glutamate transporters in the rat
cerebellar cortex.
Hioki H, Fujiyama F, Taki K, Tomioka R, Furuta T, Tamamaki N, Kaneko T.
Neuroscience. 117(1): 1-6, 2003. PubMed
Evidence that Sema3A and Sema3F regulate the migration of GABAergic neurons
in the developing neocortex.
Tamamaki N, Fujimori K, Nojyo Y, Kaneko T, Takauji R.
J Comp Neurol. 455(2): 238-248, 2003. PubMed
2002
Radial glias and radial fibers: what is the function of radial fibers?
Tamamaki N.
Anat Sci Int. 2002 Mar;77(1):2-11. Review. PubMed
Differential localization of high- and low-molecular-weight variants of
microtubule-associated protein 2 in the developing rat telencephalon.
Fujimori K, Takauji R, Tamamaki N.
J Comp Neurol. 449(4): 330-342, 2002. PubMed
2001
In vivo transduction of central neurons using recombinant Sindbis virus:
Golgi-like labeling of dendrites and axons with membrane-targeted fluorescent
proteins.
Furuta T, Tomioka R, Taki K, Nakamura K,Tamamaki N, Kaneko T.
J Histochem Cytochem. 49(12): 1497-1508, 2001. PubMed
Guidance of glial precursor cell migration by secreted cues in the developing
optic nerve.
Sugimoto Y, Taniguchi M, Yagi T, Akagi Y, Nojyo Y, Tamamaki N.
Development. 128(17): 3321-3330, 2001. PubMed
Radial glia is a progenitor of neocortical neurons in the developing cerebral
cortex.
Tamamaki N, Nakamura K, Okamoto K, Kaneko T. Neurosci Res. 41(1): 51-60, 2001. PubMed
Distribution of preganglionic terminals in the cervical sympathetic ganglia
detected by the expression of c-Fos like protein after electric stimulation
of the ventral root.
Asamoto K, Tamamaki N, Nojyo Y. Kaibogaku Zasshi. 76(3): 303-311, 2001.PubMed
Cell migration from the corticostriatal angle to the basal telencephalon
in rat embryos. Tamamaki N, Nakamura K, Kaneko T. Neuroreport. 12(4): 775-780, 2001. PubMed
2000
Neurons in Golgi-stain-like images revealed by GFP-adenovirus infection
in vivo.
Tamamaki N, Nakamura K, Furuta T, Asamoto K, Kaneko T.
Neurosci Res. 38(3): 231-236, 2000. PubMed
▼Microarray
single-cell microarray analysis
All rights reserved Ver 1.05 2007/12/19
Protocol for single-cell microarray analysis v1.05
Esumi et al. Neurosci Res. 2008