▼Purpose & Project

研究目的
大脳皮質は、記憶、情動、思考、加えて行動など、全ての中枢神経活動を司る。大脳皮質の神経活動は、二種類の構成要素である興奮性神経細胞と抑制性神経細胞のバランスの上に成り立っていている。抑制性神経細胞の働きは、神経活動のバランスを取るのみではなく、適時その抑制を緩めることに より、興奮性神経細胞の活動を高める。大脳皮質が中枢神経系の頂点にある様に、抑制性神経細胞は興奮性神経細胞の活動を支配し、興奮性神経細胞を支配する抑制性神経細胞は、他の抑制性神経細胞により支配され、全体として階層性支配様式を取っている。本教室では、中枢神経系の頂点に立つ大脳皮質抑制性神経細胞を多角的に理解すべく、分子生物学的、発生学的、形態学的観点から、その多様性と神経活動制御様式の研究を進めている。


研究プロジェクト
1. 大脳皮質GABA作動性神経細胞の産生及び分化 その多様性を生み出す分子メカニズム
2.大脳皮質興奮性神経細胞の産生
3.大脳皮質構成神経細胞数の比率を調節する分子メカニズム
4.投射型大脳皮質GABA作動性神経細胞の働き
5.胎児期および成体脳の神経幹細胞の分化制御機構
6.グリア細胞の脳高次機能における役割


▼Recent Activity

▼Cortical Development in Chania [Invited talk]2011年5月19-22日


▼Publications


2015

Neural stem cells and neuro/gliogenesis in the central nervous system: understanding the structural and functional plasticity of the developing, mature, and diseased brain.
Yamaguchi M1, Seki T2, Imayoshi I3, Tamamaki N4, Hayashi Y5, Tatebayashi Y6, Hitoshi S5.[Epub ahead of print] PubMed


Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice.
Andrews WD, Davidson K, Tamamaki N, Ruhrberg C, Parnavelas JG.
J Comp Neurol. 2015 May 15. doi: 10.1002/cne.23806. [Epub ahead of print]PubMed


Glutamate Decarboxylase 67 Deficiency in a Subset of GABAergic Neurons Induces Schizophrenia-Related Phenotypes.
Fujihara K, Miwa H, Kakizaki T, Kaneko R, Mikuni M, Tanahira C, Tamamaki N, Yanagawa Y.
Neuropsychopharmacology. 2015 Apr 23. doi: 10.1038/npp.2015.117. [Epub ahead of print]PubMed

Specification of select hypothalamic circuits and innate behaviors by the embryonic patterning gene dbx1.
Sokolowski K, Esumi S, Hirata T, Kamal Y, Tran T, Lam A, Oboti L, Brighthaupt SC, Zaghlula M, Martinez J, Ghimbovschi S, Knoblach S, Pierani A, Tamamaki N, Shah NM, Jones KS, Corbin JG.
Neuron. 2015 Apr 22;86(2):403-16. doi: 10.1016/j.neuron.2015.03.022. Epub 2015 Apr 9.PubMed


Contribution of parvalbumin and somatostatin-expressing GABAergic neurons to slow oscillations and the balance in beta-gamma oscillations across cortical layers.
Kuki T, Fujihara K, Miwa H, Tamamaki N, Yanagawa Y, Mushiake H.
Front Neural Circuits. 2015 Feb 3;9:6. doi: 10.3389/fncir.2015.00006. eCollection 2015.PubMed



2013

Birthdate study of GABAergic neurons in the lumbar spinal cord of the glutamic acid decarboxylase 67-green fluorescent protein knock-in mouse.
Huang J, Chen J, Wang W, Wei YY, Cai GH, Tamamaki N, Li YQ, Wu SX.
Front Neuroanat. 2013 Dec 9;7:42. doi: 10.3389/fnana.2013.00042. eCollection 2013.PubMed


Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated sudden death in a mouse model of Dravet syndrome.
Ogiwara I, Iwasato T, Miyamoto H, Iwata R, Yamagata T, Mazaki E, Yanagawa Y,Tamamaki N, Hensch TK, Itohara S, Yamakawa K.
Hum Mol Genet. 2013 Aug 6. [Epub ahead of print] PubMed


Voltage drives diverse endocannabinoid signals to mediate striatal microcircuit-specific plasticity.
Mathur BN, Tanahira C,Tamamaki N, Lovinger DM. Nat Neurosci. 2013 Jul 28. [Epub ahead of print] PubMed


Limk2 mediates semaphorin signalling in cortical interneurons migrating through the subpallium.
Andrews WD, Zito A, Memi F, Jones G, Tamamaki N, and Parnavelas JG.
Biol Open. 2013 15;2(3):277-282.PubMed


Amygdala kindling induces nestin expression in the leptomeninges of the neocortex.
Shogo Ninomiya, Shigeyuki Esumi, Kunimasa Ohta, Takaichi Fukuda, Tetsufumi Ito, Itaru Imayoshi, Ryoichiro Kageyama, Toshio Ikeda, Shigeyoshi Itohara, and Nobuaki Tamamaki
Neurosci Res. 2013 75:121-129.PubMed

 


2012
Identification of optogenetically activated striatal medium spiny neurons by npas4 expression.
Bepari AK, Sano H, Nobuaki Tamamaki, Nambu A, Tanaka KF, Takebayashi H.
PLoS One 2012;7(12):e52783 PubMed


Visualization of odor-induced neuronal activity by immediate early gene expression.
Bepari AK, Watanabe K, Yamaguchi M, Nobuaki Tamamaki, and Hirohide Takebayashi
BMC Neurosci. 2012 13, 140. PubMed


Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation.
Noriyoshi Usui, Keisuke Watanabe, Katsuhiko Ono, Koichi Tomita, Nobuaki Tamamaki, Kazuhiro Ikenaka and Hirohide Takebayashi
Development 2012 139,1125-1132. PubMed

 


2011
Dpy19L1, a multi-transmembrane protein, regulates radial migration of glutamatergic neurons in the developing cerebral cortex.
Keisuke Watanabe, Hirohide Takebayashi, Asim K. Bepari, Shigeyuki Esumi, Yuchio Yanagawa and Nobuaki Tamamaki
Development 2011 138, 4979-4990. PubMed


Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon
Shengxi Wu, Shigeyuki Esumi, Keisuke Watanabe, Jing Chen, Kouichi C.Nakamura, Kazuhiro Nakamura, Kouhei Kometani, Nagahiro Minato, Yuchio Yanagawa, Kaori Akashi, Kenji Sakimura, Takeshi Kaneko and Nobuaki Tamamaki.
Development 2011 138, 2499-2509 PubMed

 


2010
Long-range GABAergic connections distributed throughout the neocortex and their possible function.
Tamamaki, N. & Tomioka, R.
Front Neurosci. Focused Review 4: 202, 2010 PubMed


Cortical GABAergic Neurons: Stretching it Remarks, Main Conclusions and Discussion.
Clancy B, Defelipe J, Espinosa A, Fairen A, Jinno S, Kanold P, Luhmann HJ, Rockland KS, Tamamaki N, Yan XX.
Front Neuroanat.4:7, 2010. PubMed


Draxin, a repulsive axon guidance protein, is involved in hippocampal development.
Zhang S, Su Y, Shinmyo Y, Islam SM, Naser IB, Ahmed G, TamamakiN, Tanaka H*
Neuroscience research 66: 53-61, 2010. PubMed

 


2009
Subtypes of GABAergic neurons project axons in the neocortex.
Higo S, Akashi K, Sakimura K, Tamamaki N.
Front Neuroanat. 3: 25 2009. PubMed


Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD(67)-GFP knock-in mice.
Wang YY, Wei YY, Huang J, Wang W, Tamamaki N, Li YQ, Wu SX.
J Chem Neuroanat. 38(1): 75-81, 2009. PubMed


Parvalbumin neurons in the forebrain as revealed by parvalbumin-Cre transgenic mice.
Tanahira C, Higo S, Watanabe K, Tomioka R, Ebihara S, Kaneko T, Tamamaki N.
Neurosci Res. 63(3): 213-223, 2009. PubMed

 


2008
Rapid integration of young newborn dentate gyrus granule cells in the adult hippocampal circuitry.
Ide Y, Fujiyama F, Okamoto-Furuta K, Tamamaki N, Kaneko T, Hisatsune T
Eur J Neurosci. 28: 2381-2392, 2008. PubMed


Noradrenergic excitation of a subpopulation of GABAergic cells in the basolateral amygdala via both activation of nonselective cationic conductance and supression of resting K+ conductance: A study using GAD67-GFP knock-in mice.
Kaneko K, Tamamaki N, Owada H, Kakizaki T, Kume N, Totsuka M, Yamamoto T, Yawo H, Yagi T, Obata K, Yanagawa Y.
Neuroscience. 154(4): 781-797, 2008. PubMed


Excess Salt Causes Cerebral Neuronal Apoptosis and Inflammation in Stroke-Prone Hypertensive Rats Through Angiotensin II-Induced NADPH Oxidase Activation.
Yamamoto E, Tamamaki N, Nakamura T, Kataoka K, Tokutomi Y, Dong YF, Fukuda M, Matsuba S, Ogawa H, Kim-Mitsuyama S.
Stroke. 39(11): 3049-3056, 2008. PubMed


5-HT(3A) receptor subunit is expressed in a subpopulation of GABAergic and enkephalinergic neurons in the mouse dorsal spinal cord.
Huang J, Wang YY, Wang W, Li YQ, Tamamaki N, Wu SX.
Neurosci Lett. 441(1): 1-6, 2008. PubMed


The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse.
Hasegawa S, Hamada S, Kumode Y,Esumi S, Katori S, Fukuda E, Uchiyama Y, Hirabayashi T, Mombaerts P, Yagi T.
Mol Cell Neurosci. 38(1): 66-79, 2008. PubMed


Novel strategy to selectively label excitatory and inhibitory neurons in the cerebral cortex of mice.
Basu K, Gravel C, Tomioka R, Kaneko T,Tamamaki N, Sik A.
J Neurosci Methods. 170(2): 212-219, 2008. PubMed


Method for single-cell microarray analysis and application to gene-expression profiling of GABAergic neuron progenitors.
Esumi S, Wu SX, Yanagawa Y, Obata K, Sugimoto Y, Tamamaki N.
Neurosci Res. 60(4): 439-451, 2008. PubMed

 


2007
Anomaly with no right ventral root at the seventh cervical segment in humans: gross anatomical and neuroanatomical study.
Higo S, Koizumi M, Kawai K, Honma S, Tokiyoshi A, Tamamaki N, Kodama K.
Anat Sci Int. 82(3): 133-138, 2007. PubMed


Long-range GABAergic projection neurons in the cat neocortex.
Higo S, Udaka N, Tamamaki N.
J Comp Neurol. 503(3): 421-431, 2007. PubMed



2006
Dorsally derived netrin 1 provides an inhibitory cue and elaborates the 'waiting period' for primary sensory axons in the developing spinal cord.
Watanabe K, Tamamaki N, Furuta T, Ackerman SL, Ikenaka K, Ono K.
Development. 133(7): 1379-1387, 2006. PubMed


In vivo function of Rnd2 in the development of neocortical pyramidal neurons.
Nakamura K, Yamashita Y, Tamamaki N, Katoh H, Kaneko T, Negishi M.
Neurosci Res. 54(2): 149-153, 2006. PubMed


Split single-cell RT-PCR analysis of Purkinje cells.
Esumi S, Kaneko R, Kawamura Y, Yagi T.
Nat Protoc. 1(4): 2143-2151, 2006. PubMed


Allelic gene regulation of Pcdh-alpha and Pcdh-gamma clusters involving both monoallelic and biallelic expression in single Purkinje cells.
Kaneko R, Kato H, Kawamura Y, Esumi S, Hirayama T, Hirabayashi T, Yagi T.
J Biol Chem. 281(41): 30551-30560, 2006. PubMed


Predominant expression of rTERTb, an inactive TERT variant, in the adult rat brain.
Kaneko R, Esumi S, Yagi T, Hirabayashi T.
Protein Pept Lett. 13(1): 59-65, 2006. PubMed

 

 

2005
Pyramidal neurons of upper cortical layers generated by NEX-positive progenitor cells in the subventricular zone.
Wu SX, Goebbels S, Nakamura K, Nakamura K, Kometani K, Minato N, Kaneko T, Nave KA, Tamamaki N.
Proc Natl Acad Sci U S A. 102(47): 17172-17177, 2005. PubMed


Monoallelic yet combinatorial expression of variable exons of the protocadherin-alpha gene cluster in single neurons.
Esumi S, Kakazu N, Taguchi Y, Hirayama T, Sasaki A, Hirabayashi T, Koide T, Kitsukawa T, Hamada S, Yagi T.
Nat Genet. 37(2): 171-176, 2005. PubMed


Developmental pluripotency of the nuclei of neurons in the cerebral cortex of juvenile mice.
Osada T, Tamamaki N, Song SY, Kakazu N, Yamazaki Y, Makino H, Sasaki A, Hirayama T, Hamada S, Nave KA, Yanagimachi R, Yagi T.
J Neurosci. 25(37): 8368-8374, 2005. PubMed


Mechanisms of neuropeptide Y, peptide YY, and pancreatic polypeptide inhibition of identified green fluorescent protein-expressing GABA neurons in the hypothalamic neuroendocrine arcuate nucleus.
Acuna-Goycolea C, Tamamaki N, Yanagawa Y, Obata K, van den Pol AN.
J Neurosci. 25(32): 7406-7419, 2005. PubMed


Demonstration of long-range GABAergic connections distributed throughout the mouse neocortex.
Tomioka R, Okamoto K, Furuta T, Fujiyama F, Iwasato T, Yanagawa Y, Obata K, Kaneko T, Tamamaki N.
Eur J Neurosci. 21(6): 1587-1600, 2005. PubMed


Neurogenesis in the ependymal layer of the adult rat 3rd ventricle.
Xu Y, Tamamaki N, Noda T, Kimura K, Itokazu Y, Matsumoto N, Dezawa M, Ide C.
Exp Neurol. 192(2): 251-264, 2005. PubMed


Origin of the neocortical subependymal cells speculated by Emx1 and GAD67 expression.
Tamamaki N.
Chem Senses. 30 Suppl 1: i111-112, 2005. PubMed



2003
Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
Tamamaki N, Yanagawa Y, Tomioka R, Miyazaki J, Obata K, Kaneko T.
J Comp Neurol. 467(1): 60-79, 2003. PubMed


Changes of immunocytochemical localization of vesicular glutamate transporters in the rat visual system after the retinofugal denervation.
Fujiyama F, Hioki H, Tomioka R, Taki K, Tamamaki N, Nomura S, Okamoto K, Kaneko T.
J Comp Neurol. 465(2): 234-249, 2003. PubMed


Differential distribution of vesicular glutamate transporters in the rat cerebellar cortex.
Hioki H, Fujiyama F, Taki K, Tomioka R, Furuta T, Tamamaki N, Kaneko T.
Neuroscience. 117(1): 1-6, 2003. PubMed


Evidence that Sema3A and Sema3F regulate the migration of GABAergic neurons in the developing neocortex.
Tamamaki N, Fujimori K, Nojyo Y, Kaneko T, Takauji R.
J Comp Neurol. 455(2): 238-248, 2003. PubMed



2002
Radial glias and radial fibers: what is the function of radial fibers?
Tamamaki N.
Anat Sci Int. 2002 Mar;77(1):2-11. Review. PubMed


Differential localization of high- and low-molecular-weight variants of microtubule-associated protein 2 in the developing rat telencephalon.
Fujimori K, Takauji R, Tamamaki N.
J Comp Neurol. 449(4): 330-342, 2002. PubMed



2001
In vivo transduction of central neurons using recombinant Sindbis virus: Golgi-like labeling of dendrites and axons with membrane-targeted fluorescent proteins.
Furuta T, Tomioka R, Taki K, Nakamura K,Tamamaki N, Kaneko T.
J Histochem Cytochem. 49(12): 1497-1508, 2001. PubMed


Guidance of glial precursor cell migration by secreted cues in the developing optic nerve.
Sugimoto Y, Taniguchi M, Yagi T, Akagi Y, Nojyo Y, Tamamaki N.
Development. 128(17): 3321-3330, 2001. PubMed


Radial glia is a progenitor of neocortical neurons in the developing cerebral cortex.
Tamamaki N, Nakamura K, Okamoto K, Kaneko T. Neurosci Res. 41(1): 51-60, 2001. PubMed


Distribution of preganglionic terminals in the cervical sympathetic ganglia detected by the expression of c-Fos like protein after electric stimulation of the ventral root.
Asamoto K, Tamamaki N, Nojyo Y. Kaibogaku Zasshi. 76(3): 303-311, 2001.PubMed


Cell migration from the corticostriatal angle to the basal telencephalon in rat embryos. Tamamaki N, Nakamura K, Kaneko T. Neuroreport. 12(4): 775-780, 2001. PubMed



2000
Neurons in Golgi-stain-like images revealed by GFP-adenovirus infection in vivo.
Tamamaki N, Nakamura K, Furuta T, Asamoto K, Kaneko T.
Neurosci Res. 38(3): 231-236, 2000. PubMed

 

 

 

▼Microarray

single-cell microarray analysis


        All rights reserved  Ver 1.05 2007/12/19

       Protocol for single-cell microarray analysis v1.05
       Esumi et al. Neurosci Res. 2008